Mosquito Mounting Tutorial — Larval Stage

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Section II: WRBU Mosquito Larva Mounting Protocol

Maceration

The cuticle must be cleared so that all characters are visible when the specimen is mounted. Mosquito larvae can be cleared using a variety of chemicals and it is important to monitor temperature and timing of exposure. Because ... clear quickly and easily ... as the length of time in NaOH increases, care should be taken to avoid over-clearing. If ... is difficult to clear, use a probe to create a small hole, at an angle, where the ... and ... join. Because the ... may clear more slowly than the ..., it may be necessary to leave the ... in the NaOH for an extra 5 to 10 minutes until it is mostly clear and not .... Record clear times for ... and ... in lab notebook.


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